Hotspot

rbcL

LSC · 54677-56110 · 1434 bp

Back to Cynara

Pi 0.00321
MarkerSeek score 45.90
Primer available yes
Label rbcL

Feature Metadata

label_name
rbcL
feature_type
gene
region
LSC
length_bp
1434
variable_sites
8
pi
0.00321
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL_p1 TCATGAGTTGTAGGGAGGGA TATGCATGTTGGATTCGGCT 50.0 45.0 57.1 57.7 1537 bp 1.0 70.1
rbcL_p2 ACGCCTAATTCATGTCGAGT TATGCATGTTGGATTCGGCT 45.0 45.0 57.0 57.7 1585 bp 1.0 69.9
rbcL_p3 TTCATGAGTTGTAGGGAGGGA TATGCATGTTGGATTCGGCT 47.6 45.0 57.8 57.7 1538 bp 1.0 68.0
rbcL_p4 AACGCCTAATTCATGTCGAGT TATGCATGTTGGATTCGGCT 42.9 45.0 57.8 57.7 1586 bp 1.0 67.7
rbcL_p5 TCATGAGTTGTAGGGAGGGAT TATGCATGTTGGATTCGGCT 47.6 45.0 57.6 57.7 1537 bp 1.0 66.3