Hotspot

petD

LSC · 75997-77184 · 1188 bp

Pi 0.000673

MarkerSeek score 44.30

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1188
variable_sites: 2
pi: 0.000673
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ATTTCCCTTGAACTCCGCAA	TGAAGCGACTGTTCCCTAGA	45.0	50.0	57.7	58.1	1322 bp	1.0	77.2
petD_p2	TTTCCCTTGAACTCCGCAAT	TGAAGCGACTGTTCCCTAGA	45.0	50.0	57.7	58.1	1321 bp	1.0	77.2
petD_p3	ATTTCCCTTGAACTCCGCAA	CTTTGAAGCGACTGTTCCCT	45.0	50.0	57.7	58.1	1325 bp	1.0	76.9
petD_p4	TTTCCCTTGAACTCCGCAAT	CTTTGAAGCGACTGTTCCCT	45.0	50.0	57.7	58.1	1324 bp	1.0	76.9
petD_p5	TTTCCCTTGAACTCCGCAAT	ACTTTGAAGCGACTGTTCCC	45.0	50.0	57.7	58.4	1325 bp	1.0	74.6