Hotspot

rpl32-ndhF

SSC · 124111-125160 · 1050 bp

Back to Cynara

Pi 0.000952
MarkerSeek score 45.10
Primer available yes
Label rpl32-ndhF

Feature Metadata

label_name
rpl32-ndhF
feature_type
igs
region
SSC
length_bp
1050
variable_sites
2
pi
0.000952
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-ndhF_p1 CCGCCCAATATCCTTTCCTT GTTGCCGTCGGAAAAAGAAG 50.0 50.0 57.6 58.0 1224-1230 bp 1.0 77.3
rpl32-ndhF_p2 CCGCCCAATATCCTTTCCTT TGCCGTCGGAAAAAGAAGAA 50.0 45.0 57.6 58.0 1222-1228 bp 1.0 77.3
rpl32-ndhF_p3 CCGCCCAATATCCTTTCCTT TTGCCGTCGGAAAAAGAAGA 50.0 45.0 57.6 58.0 1223-1229 bp 1.0 77.3
rpl32-ndhF_p4 TGACAAAGCTTTTAACGCCG GTTGCCGTCGGAAAAAGAAG 45.0 50.0 57.6 58.0 1241-1247 bp 1.0 77.1
rpl32-ndhF_p5 TGACAAAGCTTTTAACGCCG TGCCGTCGGAAAAAGAAGAA 45.0 45.0 57.6 58.0 1239-1245 bp 1.0 77.0