Hotspot

psaJ-rpl33

LSC · 66714-67134 · 421 bp

Back to Cynara

Pi 0.00665
MarkerSeek score 32.30
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
421
variable_sites
6
pi
0.00665
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
32.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 ACATATCTTTCCGTGGCACC TCGCACATCTTTACCCTTGG 50.0 50.0 58.0 57.9 565-574 bp 1.0 79.7
psaJ-rpl33_p2 ACATATCTTTCCGTGGCACC CACATCTTTACCCTTGGCCA 50.0 50.0 58.0 57.8 562-571 bp 1.0 78.8
psaJ-rpl33_p3 TATGGTTCGCTTCTTTGGCA TCGCACATCTTTACCCTTGG 45.0 50.0 57.8 57.9 531-540 bp 1.0 78.5
psaJ-rpl33_p4 TGGCACCGGTACTAAGTACT TCGCACATCTTTACCCTTGG 50.0 50.0 57.8 57.9 552-561 bp 1.0 78.2
psaJ-rpl33_p5 TCGCTTCTTTGGCAGGTTTA TCGCACATCTTTACCCTTGG 45.0 50.0 57.7 57.9 525-534 bp 1.0 77.9