Hotspot

psbE-petL

LSC · 64191-65470 · 1280 bp

Back to Cynara

Pi 0.00234
MarkerSeek score 45.50
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1280
variable_sites
6
pi
0.00234
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA GTCGTATCTTGCTCAGACCA 50.0 50.0 57.9 57.1 1472-1477 bp 1.0 72.4
psbE-petL_p2 AGAACGTTCTCCTGTGCTTC GTCGTATCTTGCTCAGACCA 50.0 50.0 57.8 57.1 1395-1400 bp 1.0 71.6
psbE-petL_p3 ACGAATAACCAACCCGCAAT GTCGTATCTTGCTCAGACCA 45.0 50.0 58.2 57.1 1481-1486 bp 1.0 71.5
psbE-petL_p4 TGCTGACGAATAACCAACCC GTCGTATCTTGCTCAGACCA 50.0 50.0 58.2 57.1 1486-1491 bp 1.0 71.4
psbE-petL_p5 CGAATAACCAACCCGCAATG GTCGTATCTTGCTCAGACCA 50.0 50.0 57.8 57.1 1480-1485 bp 1.0 71.3