Hotspot

accD-psaI

LSC · 58107-58776 · 670 bp

Back to Cynara

Pi 0.00418
MarkerSeek score 32.50
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
670
variable_sites
5
pi
0.00418
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
32.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 AATGCCTACATTGCGTTTGC AAAGAAGCCATTGCGATTGC 45.0 45.0 58.0 58.0 942 bp 1.0 80.5
accD-psaI_p2 AATGCCTACATTGCGTTTGC CCATTCGTCAATTCGGGTCT 45.0 50.0 58.0 58.0 1160-1161 bp 1.0 80.2
accD-psaI_p3 AATGCCTACATTGCGTTTGC ATAAAAAGACCCGCGATCCC 45.0 50.0 58.0 58.0 1608-1609 bp 1.0 80.1
accD-psaI_p4 AATGCCTACATTGCGTTTGC AGATTTTGTCCCATCGGCTC 45.0 50.0 58.0 58.0 995 bp 1.0 80.1
accD-psaI_p5 AATGCCTACATTGCGTTTGC GATTTTGTCCCATCGGCTCT 45.0 50.0 58.0 58.0 994 bp 1.0 80.1