Hotspot

ndhC-trnV-UAC

LSC · 49808-50932 · 1125 bp

Back to Cynara

Pi 0.00089
MarkerSeek score 52.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1125
variable_sites
2
pi
0.00089
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GTCCTTTGCTAATCGGAGCT CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.7 58.1 1271-1279 bp 1.0 76.9
ndhC-trnV-UAC_p2 CGGTCCTTTGCTAATCGGAG CCGAGAAAGTCTACGGTTCG 55.0 55.0 58.4 58.1 1273-1281 bp 1.0 76.0
ndhC-trnV-UAC_p3 CTCCGGTCCTTTGCTAATCG CCGAGAAAGTCTACGGTTCG 55.0 55.0 58.4 58.1 1276-1284 bp 1.0 76.0
ndhC-trnV-UAC_p4 GGTCCTTTGCTAATCGGAGC CCGAGAAAGTCTACGGTTCG 55.0 55.0 58.7 58.1 1272-1280 bp 1.0 73.8
ndhC-trnV-UAC_p5 GTCCTTTGCTAATCGGAGCT GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.7 58.7 1256-1264 bp 1.0 72.2