Hotspot

trnF-GAA-ndhJ

LSC · 47464-48136 · 673 bp

Back to Cynara

Pi 0.00119
MarkerSeek score 48.40
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
673
variable_sites
2
pi
0.00119
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA TGGATAGGATGGCCTTTACG 50.0 50.0 58.1 56.8 776 bp 1.0 70.0
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA GGATAGGATGGCCTTTACGT 50.0 50.0 58.1 56.8 775 bp 1.0 70.0
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA TGAAAGTTGGATAGGATGGCC 50.0 47.6 58.1 57.7 783 bp 1.0 69.4
trnF-GAA-ndhJ_p4 CCTCGTGTCACCAGTTCAAA TTGGATAGGATGGCCTTTACG 50.0 47.6 58.1 57.5 777 bp 1.0 67.9
trnF-GAA-ndhJ_p5 CCTCGTGTCACCAGTTCAAA TGGATAGGATGGCCTTTACGT 50.0 47.6 58.1 58.6 776 bp 1.0 67.0