Hotspot

ycf3-trnS-GGA

LSC · 43639-44539 · 901 bp

Back to Cynara

Pi 0.000666
MarkerSeek score 48.30
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
901
variable_sites
1
pi
0.000666
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CATTTATCCGCGATCTAGGCA ACGGAAAGAGAGGGATTCGA 47.6 50.0 58.0 58.2 985-1018 bp 1.0 73.2
ycf3-trnS-GGA_p2 CATTTATCCGCGATCTAGGCA TTCCAATGCTACGCCTTCAA 47.6 45.0 58.0 57.8 935-968 bp 1.0 73.0
ycf3-trnS-GGA_p3 CATTTATCCGCGATCTAGGCA GGATTCGAACCCTCGGTAAG 47.6 55.0 58.0 57.8 973-1006 bp 1.0 72.7
ycf3-trnS-GGA_p4 CATTTATCCGCGATCTAGGCA AGTTCCAATGCTACGCCTTC 47.6 50.0 58.0 58.3 937-970 bp 1.0 72.4
ycf3-trnS-GGA_p5 CATTTATCCGCGATCTAGGCA CCTCGGTAAGCAAAAGCCTA 47.6 50.0 58.0 57.6 963-996 bp 1.0 71.3