Pi
0.0678
MarkerSeek score
77.80
Primer available
yes
Label
Ycf1
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| Ycf1_p1 | CCCGGAACTTCTTCGTAGTG | GAAAACCCTTCCTGCAGACA | 55.0 | 50.0 | 58.0 | 58.0 | 1186-1302 bp | 0.25 | 54.0 |
| Ycf1_p2 | CCGGAACTTCTTCGTAGTGG | GAAAACCCTTCCTGCAGACA | 55.0 | 50.0 | 58.0 | 58.0 | 1185-1301 bp | 0.25 | 54.0 |
| Ycf1_p3 | AACCCACGACCAATATGAGC | GAAAACCCTTCCTGCAGACA | 50.0 | 50.0 | 58.0 | 58.0 | 1226-1342 bp | 0.25 | 53.8 |
| Ycf1_p4 | CCCGGAACTTCTTCGTAGTG | AATGGGACGGAAAACCCTTC | 55.0 | 50.0 | 58.0 | 58.1 | 1195-1311 bp | 0.25 | 53.5 |
| Ycf1_p5 | CCGGAACTTCTTCGTAGTGG | AATGGGACGGAAAACCCTTC | 55.0 | 50.0 | 58.0 | 58.1 | 1194-1310 bp | 0.25 | 53.5 |
| Ycf1_p1 | GTCTCCCTTTTCGGTTCCTG | CGACTTGAAGCTCTCGACAA | 55.0 | 50.0 | 58.2 | 57.9 | 899-1043 bp | 1.0 | 90.1 |
| Ycf1_p2 | GGTTCCTGTTCCGATTCCAA | CGACTTGAAGCTCTCGACAA | 50.0 | 50.0 | 57.8 | 57.9 | 887-1031 bp | 1.0 | 90.0 |
| Ycf1_p3 | GTCTCCCTTTTCGGTTCCTG | TAAGTGGGCCCTATCGCTTA | 55.0 | 50.0 | 58.2 | 57.9 | 643-859 bp | 1.0 | 89.7 |
| Ycf1_p4 | TTCCTCGTCTTCCTCGTCTT | TAAGTGGGCCCTATCGCTTA | 50.0 | 50.0 | 58.1 | 57.9 | 719-785 bp | 0.375 | 63.9 |
| Ycf1_p5 | TTCCTCGTCTTCCTCGTCTT | CGACTTGAAGCTCTCGACAA | 50.0 | 50.0 | 58.1 | 57.9 | 903-969 bp | 0.375 | 63.6 |