Hotspot

Ycf1-trnN-GUU

IRa · 135101-135869 · 769 bp

Back to Cynanchum

Pi 0.0396

MarkerSeek score 76.60

Primer available yes

Label Ycf1-trnN-GUU

Feature Metadata

label_name: Ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 769
variable_sites: 72
pi: 0.0396
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
Ycf1-trnN-GUU_p1	GCCTGAACTGGGAAAAGGAA	CGGGAGAATTGATGACTGCA	50.0	50.0	58.0	58.0	853-1157 bp	0.875	82.5
Ycf1-trnN-GUU_p2	GCCTGAACTGGGAAAAGGAA	GTAAATCTTCCTCGGCCAGG	50.0	55.0	58.0	58.0	970-1274 bp	0.875	82.1
Ycf1-trnN-GUU_p3	GCCTGAACTGGGAAAAGGAA	AGAATGATGGGGATAGCGGA	50.0	50.0	58.0	58.0	1134-1438 bp	0.875	81.8
Ycf1-trnN-GUU_p4	GCCTGAACTGGGAAAAGGAA	AGATAAACGCGCCTTCACAT	50.0	45.0	58.0	58.0	1357-1661 bp	0.75	76.0
Ycf1-trnN-GUU_p5	GCCTGAACTGGGAAAAGGAA	ATAAACGCGCCTTCACATCT	50.0	45.0	58.0	58.0	1355-1659 bp	0.75	76.0