Hotspot

rpl32-trnL-UAG

SSC · 118240-119227 · 988 bp

Back to Cynanchum

Pi 0.0604
MarkerSeek score 82.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
988
variable_sites
141
pi
0.0604
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.87
markerseek_score
82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ACCTACCCCATTTGACCAGT AACCACTCGGATTGAAGAGC 50.0 50.0 58.2 57.9 2013-2019 bp 0.25 47.9
rpl32-trnL-UAG_p2 ACCTACCCCATTTGACCAGT TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 1481-1488 bp 0.25 47.9
rpl32-trnL-UAG_p3 ACCTACCCCATTTGACCAGT TAACAGTCCGGAAGTAGCGA 50.0 50.0 58.2 58.2 1960-1966 bp 0.25 47.3
rpl32-trnL-UAG_p4 ACCTACCCCATTTGACCAGT AGCACTTGACCCGGTTATTG 50.0 50.0 58.2 58.2 1924-1930 bp 0.25 47.3
rpl32-trnL-UAG_p5 ACCTACCCCATTTGACCAGT CTTGACCCGGTTATTGTGCT 50.0 50.0 58.2 58.2 1920-1926 bp 0.25 47.3