Hotspot

ndhF-rpl32

SSC · 117389-118080 · 692 bp

Back to Cynanchum

Pi 0.0579

MarkerSeek score 83.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 692
variable_sites: 96
pi: 0.0579
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTTGAGGTCGTGTGAACCAA	AAAGAGGCTCTCCCCATCTT	50.0	50.0	58.1	58.0	1775-1783 bp	0.25	50.0
ndhF-rpl32_p2	AGGAGCCACCCGTATTGATA	AAAGAGGCTCTCCCCATCTT	50.0	50.0	57.9	58.0	1296-1304 bp	0.25	49.7
ndhF-rpl32_p3	TAGGAGCCACCCGTATTGAT	AAAGAGGCTCTCCCCATCTT	50.0	50.0	57.9	58.0	1297-1305 bp	0.25	49.7
ndhF-rpl32_p4	CGTCGGCAAAATAAGAAGGC	AAAGAGGCTCTCCCCATCTT	50.0	50.0	57.8	58.0	1357-1365 bp	0.25	49.1
ndhF-rpl32_p5	AGTGGGATGAAGGGTAGGAG	AAAGAGGCTCTCCCCATCTT	55.0	50.0	57.8	58.0	1311-1319 bp	0.25	49.0