Hotspot

Ycf1-ndhF

SSC · 114858-115165 · 308 bp

Back to Cynanchum

Pi 0.172

MarkerSeek score 65.90

Primer available yes

Label Ycf1-ndhF

Feature Metadata

label_name: Ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 308
variable_sites: 109
pi: 0.172
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
Ycf1-ndhF_p1	TTCCTTTTCCCAGTTCAGGC	CAGTCTTAACGGGGTGCATT	50.0	50.0	58.0	58.2	944 bp	0.25	48.7
Ycf1-ndhF_p2	GGCTATGGAAGAAGGAACCG	CAGTCTTAACGGGGTGCATT	55.0	50.0	58.0	58.2	921 bp	0.25	48.4
Ycf1-ndhF_p3	CAATTCGGTCGTTTTGGTCG	CAGTCTTAACGGGGTGCATT	50.0	50.0	58.1	58.2	1014 bp	0.25	48.3
Ycf1-ndhF_p4	GAAGAAGGCAGCAGCAAAAG	CAGTCTTAACGGGGTGCATT	50.0	50.0	57.9	58.2	894 bp	0.25	48.3
Ycf1-ndhF_p5	AGGAACCGAGAACGAGAAGA	CAGTCTTAACGGGGTGCATT	50.0	50.0	58.1	58.2	909 bp	0.25	48.0