Hotspot

ndhC-trnV-UAC

LSC · 55115-56685 · 1571 bp

Back to Cynanchum

Pi 0.052
MarkerSeek score 77.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1571
variable_sites
163
pi
0.052
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.80
markerseek_score
77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GCCTTTGTTAATCGGGGCTA TGAGTCTAAGGGCCTCAACA 50.0 50.0 58.0 58.0 963-2115 bp 1.0 91.4
ndhC-trnV-UAC_p2 GCCTTTGTTAATCGGGGCTA GGTTGATCTAGGCCAGAAGC 50.0 55.0 58.0 58.0 855-2006 bp 1.0 91.3
ndhC-trnV-UAC_p3 CCTCCGGTAATTGTACAGGC TGAGTCTAAGGGCCTCAACA 55.0 50.0 58.1 58.0 1600-2752 bp 1.0 90.2
ndhC-trnV-UAC_p4 CTCCGGTAATTGTACAGGCC TGAGTCTAAGGGCCTCAACA 55.0 50.0 58.1 58.0 2159-2751 bp 0.25 53.4
ndhC-trnV-UAC_p5 CTCCGGTAATTGTACAGGCC GGTTGATCTAGGCCAGAAGC 55.0 55.0 58.1 58.0 2050-2642 bp 0.25 53.3