Hotspot

trnL-UAA-trnF-GAA

LSC · 52423-52740 · 318 bp

Back to Cynanchum

Pi 0.0446

MarkerSeek score 75.60

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 318
variable_sites: 34
pi: 0.0446
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.5	58.0	367-392 bp	1.0	87.4
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	403-428 bp	1.0	86.4
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAG	50.0	55.0	57.5	57.9	379-404 bp	1.0	86.1
trnL-UAA-trnF-GAA_p4	TTCAAGTCCCTCTATCCCCA	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.0	58.0	356-381 bp	1.0	83.7
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	400-425 bp	1.0	83.2