Hotspot

trnE-UUC-trnT-GGU

LSC · 35128-36208 · 1081 bp

Back to Cynanchum

Pi 0.0404

MarkerSeek score 76.60

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1081
variable_sites: 104
pi: 0.0404
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	1040-1201 bp	1.0	90.4
trnE-UUC-trnT-GGU_p2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	1038-1199 bp	1.0	90.4
trnE-UUC-trnT-GGU_p3	GATGTCCTGAACCGCTAGAC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	1037-1198 bp	1.0	89.9
trnE-UUC-trnT-GGU_p4	CTTGCTTTCCTAGTCCACCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.9	58.0	1170-1257 bp	0.375	62.4
trnE-UUC-trnT-GGU_p5	CTTGCTTTCCTAGTCCACCC	TACCGCTGAGTTAAAAGGGC	55.0	50.0	57.9	57.9	1135-1222 bp	0.375	61.7