Hotspot

trnH-GUG-psbA

LSC · 92-313 · 222 bp

Back to Cynanchum

Pi 0.0871
MarkerSeek score 77.40
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
222
variable_sites
48
pi
0.0871
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 AATGGTAAATGGGCGAACGA GCCGGTAGTAGGTATCTGGT 45.0 55.0 57.9 58.0 537 bp 0.125 44.0
trnH-GUG-psbA_p2 AATGGTAAATGGGCGAACGA TGGCCGGTAGTAGGTATCTG 45.0 55.0 57.9 58.0 539 bp 0.125 44.0
trnH-GUG-psbA_p3 AATGGTAAATGGGCGAACGA GAGCTTAGTTTCCGTCTGGG 45.0 55.0 57.9 58.0 977 bp 0.125 44.0
trnH-GUG-psbA_p4 AATGGTAAATGGGCGAACGA GGAGCTTAGTTTCCGTCTGG 45.0 55.0 57.9 58.0 978 bp 0.125 44.0
trnH-GUG-psbA_p5 AATGGTAAATGGGCGAACGA GGGAGCTTAGTTTCCGTCTG 45.0 55.0 57.9 58.0 979 bp 0.125 44.0