Hotspot

ccsA-ndhD

SSC · 112132-112309 · 178 bp

Back to Cymbopogon

Pi 0.0202

MarkerSeek score 55.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 178
variable_sites: 11
pi: 0.0202
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CCGCACTTGTAGCTTCGATA	TCCGTTGATAGGGTAGAGGT	50.0	50.0	57.8	56.9	338-341 bp	1.0	72.1
ccsA-ndhD_p2	CCGCACTTGTAGCTTCGATA	TCCGTTGATAGGGTAGAGGTT	50.0	47.6	57.8	57.6	338-341 bp	1.0	70.1
ccsA-ndhD_p3	ATTCCGCACTTGTAGCTTCG	TCCGTTGATAGGGTAGAGGT	50.0	50.0	58.6	56.9	341-344 bp	1.0	68.6
ccsA-ndhD_p4	CGAATTCCGCACTTGTAGCT	TCCGTTGATAGGGTAGAGGT	50.0	50.0	58.6	56.9	344-347 bp	1.0	68.5
ccsA-ndhD_p5	CCGCACTTGTAGCTTCGATA	ATCCGTTGATAGGGTAGAGGT	50.0	47.6	57.8	57.4	339-342 bp	1.0	68.3