Hotspot

rpl32-trnL-UAG

SSC · 110556-111008 · 453 bp

Back to Cymbopogon

Pi 0.00883

MarkerSeek score 45.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 453
variable_sites: 10
pi: 0.00883
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CAACGAGCATCCAAAACCAA	TTCGAACCGAGATGCTTGAG	45.0	50.0	57.2	58.0	573-575 bp	1.0	75.3
rpl32-trnL-UAG_p2	CAACGAGCATCCAAAACCAA	GGATTCGAACCGAGATGCTT	45.0	50.0	57.2	58.1	576-578 bp	1.0	74.9
rpl32-trnL-UAG_p3	CAACGAGCATCCAAAACCAA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.2	57.9	546-548 bp	1.0	74.4
rpl32-trnL-UAG_p4	CAACGAGCATCCAAAACCAA	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.2	57.8	539-541 bp	1.0	74.0
rpl32-trnL-UAG_p5	CAACGAGCATCCAAAACCAA	GCTTGAGCACTGCTTCCTAA	45.0	50.0	57.2	58.2	560-562 bp	1.0	73.9