Hotspot

ndhF-rpl32

SSC · 109520-110372 · 853 bp

Back to Cymbopogon

Pi 0.00427
MarkerSeek score 53.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
853
variable_sites
15
pi
0.00427
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGGAGAAGAGGA TCCAAAGATTTTTACGAATACGCT 55.0 33.3 58.1 57.3 965-976 bp 1.0 43.1
ndhF-rpl32_p2 CTGGAAGTGGGAGAAGAGGA TGACATCGAAGTACGTTTTT 55.0 35.0 58.1 53.2 937-948 bp 1.0 42.9
ndhF-rpl32_p3 CTGGAAGTGGGAGAAGAGGA TTGACATCGAAGTACGTTTT 55.0 35.0 58.1 53.2 938-949 bp 1.0 42.9
ndhF-rpl32_p4 CTGGAAGTGGGAGAAGAGGA TTTGACATCGAAGTACGTTT 55.0 35.0 58.1 53.2 939-950 bp 1.0 42.9
ndhF-rpl32_p5 CTGGAAGTGGGAGAAGAGGA TTTTGACATCGAAGTACGTT 55.0 35.0 58.1 53.2 940-951 bp 1.0 42.9