Hotspot

rps12-trnV-GAC

IRb · 95295-97050 · 1756 bp

Back to Cymbopogon

Pi 0.000342

MarkerSeek score 50.60

Primer available yes

Label rps12-trnV-GAC

Feature Metadata

label_name: rps12-trnV-GAC
feature_type: igs
region: IRb
length_bp: 1756
variable_sites: 3
pi: 0.000342
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-trnV-GAC_p1	CGTAAGGCAGAGTTGGGTTT	GATGACTTCCACCACGTCAA	50.0	50.0	58.1	57.8	1830-1841 bp	1.0	78.9
rps12-trnV-GAC_p2	CGTAAGGCAGAGTTGGGTTT	AACTGATGACTTCCACCACG	50.0	50.0	58.1	57.8	1834-1845 bp	1.0	78.8
rps12-trnV-GAC_p3	CGTAAGGCAGAGTTGGGTTT	ATGACTTCCACCACGTCAAG	50.0	50.0	58.1	57.8	1829-1840 bp	1.0	78.8
rps12-trnV-GAC_p4	CGTAAGGCAGAGTTGGGTTT	TGGTGGGGGAGTAAGTCAAA	50.0	50.0	58.1	58.2	1895-1906 bp	1.0	78.7
rps12-trnV-GAC_p5	CGTAAGGCAGAGTTGGGTTT	CTGATGACTTCCACCACGTC	50.0	55.0	58.1	58.3	1832-1843 bp	1.0	77.9