Hotspot

trnM-CAU-ycf2

IRb · 87170-88490 · 1321 bp

Back to Cymbopogon

Pi 0.000303

MarkerSeek score 49.90

Primer available yes

Label trnM-CAU-ycf2

Feature Metadata

label_name: trnM-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 1321
variable_sites: 2
pi: 0.000303
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	CCCCATATTCTAGGAGCCCA	50.0	55.0	58.0	58.0	1404-1426 bp	1.0	81.0
trnM-CAU-ycf2_p2	GAGCCAGTTCCAATAGACCG	CCCCATATTCTAGGAGCCCA	55.0	55.0	58.1	58.0	1478-1500 bp	1.0	80.5
trnM-CAU-ycf2_p3	GCCAGTTCCAATAGACCGTT	CCCCATATTCTAGGAGCCCA	50.0	55.0	57.9	58.0	1476-1498 bp	1.0	80.1
trnM-CAU-ycf2_p4	GTTGGGCGCTTTAACCATTC	TATTCAGCGTACCTGTCCCT	50.0	50.0	58.0	57.8	1449-1471 bp	1.0	80.1
trnM-CAU-ycf2_p5	GAGCCAGTTCCAATAGACCG	TATTCAGCGTACCTGTCCCT	55.0	50.0	58.1	57.8	1523-1545 bp	1.0	79.6