Hotspot

ndhC-trnM-CAU

LSC · 54426-56187 · 1762 bp

Back to Cymbopogon

Pi 0.00156

MarkerSeek score 52.80

Primer available yes

Label ndhC-trnM-CAU

Feature Metadata

label_name: ndhC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 1762
variable_sites: 13
pi: 0.00156
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnM-CAU_p1	CTAACCGGGGCTAAAAGTCC	CCCGCCGTATGAAAGCAATA	55.0	50.0	58.0	58.4	1905-1969 bp	1.0	78.4
ndhC-trnM-CAU_p2	CTAACCGGGGCTAAAAGTCC	CCGCCGTATGAAAGCAATAC	55.0	50.0	58.0	57.3	1904-1968 bp	1.0	76.4
ndhC-trnM-CAU_p3	TAACCGGGGCTAAAAGTCCT	CCCGCCGTATGAAAGCAATA	50.0	50.0	58.3	58.4	1904-1968 bp	1.0	76.0
ndhC-trnM-CAU_p4	CTAACCGGGGCTAAAAGTCC	TTTGAACCAATGACTCCCGC	55.0	50.0	58.0	58.8	1920-1984 bp	1.0	75.7
ndhC-trnM-CAU_p5	CTAACCGGGGCTAAAAGTCC	AGCGAAGTAAGTCCAATGCT	55.0	45.0	58.0	57.2	1977-2041 bp	1.0	75.5