Hotspot

trnT-UGU-trnF-GAA

LSC · 50389-52104 · 1716 bp

Back to Cymbopogon

Pi 0.00227

MarkerSeek score 52.40

Primer available yes

Label trnT-UGU-trnF-GAA

Feature Metadata

label_name: trnT-UGU-trnF-GAA
feature_type: igs
region: LSC
length_bp: 1716
variable_sites: 17
pi: 0.00227
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnF-GAA_p1	ATTTGAACCGATGACCCTCG	TTTGAACTGGTGACACGAGG	50.0	50.0	58.0	58.1	1807-1840 bp	1.0	82.1
trnT-UGU-trnF-GAA_p2	ATTTGAACCGATGACCCTCG	CTGTGCCAGGAACCAGATTT	50.0	50.0	58.0	58.1	1824-1857 bp	1.0	81.8
trnT-UGU-trnF-GAA_p3	ATTTGAACCGATGACCCTCG	GTGCCAGGAACCAGATTTGA	50.0	50.0	58.0	58.1	1822-1855 bp	1.0	81.7
trnT-UGU-trnF-GAA_p4	CGATGACCCTCGCATTACAA	TTTGAACTGGTGACACGAGG	50.0	50.0	58.1	58.1	1799-1832 bp	1.0	81.7
trnT-UGU-trnF-GAA_p5	CGATGACCCTCGCATTACAA	CTGTGCCAGGAACCAGATTT	50.0	50.0	58.1	58.1	1816-1849 bp	1.0	81.4