Hotspot

trnC-GCA-rpoB

LSC · 22361-23578 · 1218 bp

Back to Cymbopogon

Pi 0.00316

MarkerSeek score 57.00

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1218
variable_sites: 16
pi: 0.00316
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	CTTCTCAAGTTCTTCCGCCA	50.0	50.0	58.1	57.8	1362-1382 bp	1.0	80.6
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	TCTCAAGTTCTTCCGCCAAG	50.0	50.0	58.1	57.8	1360-1380 bp	1.0	80.6
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	ACTTCTCAAGTTCTTCCGCC	50.0	50.0	58.1	57.8	1363-1383 bp	1.0	80.6
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	TTCCGCCAAGCCTTGATTAA	50.0	45.0	58.1	57.8	1350-1370 bp	1.0	80.3
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	CTTCCGCCAAGCCTTGATTA	50.0	50.0	58.1	58.2	1351-1371 bp	1.0	80.0