Hotspot

trnT-GGU-trnE-UUC

LSC · 18875-19421 · 547 bp

Back to Cymbopogon

Pi 0.00861
MarkerSeek score 59.60
Primer available yes
Label trnT-GGU-trnE-UUC

Feature Metadata

label_name
trnT-GGU-trnE-UUC
feature_type
igs
region
LSC
length_bp
547
variable_sites
22
pi
0.00861
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnE-UUC_p1 AGGCATAAGTCATCGGTTCA TGTCCTGAACCACTAGACGA 45.0 50.0 56.6 57.7 609-617 bp 1.0 72.3
trnT-GGU-trnE-UUC_p2 AGCCCTTTTAACTCAGTGGT TGTCCTGAACCACTAGACGA 45.0 50.0 56.7 57.7 646-654 bp 1.0 72.2
trnT-GGU-trnE-UUC_p3 AGGCATAAGTCATCGGTTCA GCTGCCTCCTTGAAAGAGAG 45.0 55.0 56.6 58.3 630-638 bp 1.0 72.2
trnT-GGU-trnE-UUC_p4 AGCCCTTTTAACTCAGTGGT GCTGCCTCCTTGAAAGAGAG 45.0 55.0 56.7 58.3 667-675 bp 1.0 72.1
trnT-GGU-trnE-UUC_p5 TGGTAAGGCATAAGTCATCGG GCTGCCTCCTTGAAAGAGAG 47.6 55.0 57.5 58.3 635-643 bp 1.0 71.4