Hotspot

ccsA-ndhD

SSC · 117900-118193 · 294 bp

Back to Cymbidium

Pi 0.0519

MarkerSeek score 70.80

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 294
variable_sites: 126
pi: 0.0519
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AGTTATGGTTCATTCCAATTACT	TCGGTTGACAAGGTACAAGT	30.4	45.0	53.7	56.7	355-377 bp	0.863636	46.5
ccsA-ndhD_p2	TGGTTCATTCCAATTACTATCT	TCGGTTGACAAGGTACAAGT	31.8	45.0	52.6	56.7	350-372 bp	0.863636	46.5
ccsA-ndhD_p3	AGTTATGGTTCATTCCAATTACT	TCGGTTGACAAGGTACAAGTT	30.4	42.9	53.7	57.5	355-377 bp	0.863636	46.5
ccsA-ndhD_p4	TGGTTCATTCCAATTACTATCT	TCGGTTGACAAGGTACAAGTT	31.8	42.9	52.6	57.5	350-372 bp	0.863636	46.5
ccsA-ndhD_p5	ATGGTTCATTCCAATTACTATCT	TCGGTTGACAAGGTACAAGT	30.4	45.0	53.2	56.7	351-373 bp	0.863636	46.5