Hotspot

ndhF-rpl32

SSC · 114901-115850 · 950 bp

Back to Cymbidium

Pi 0.183
MarkerSeek score 71.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
950
variable_sites
524
pi
0.183
unique_haplotype_count
41
primer_available
yes
alignment_reliability
0.58
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TATCTTCCCTGTCCCAAGCA AAGTTTCGTTCTCAGTCGGG 50.0 50.0 58.0 57.8 1928-2836 bp 0.181818 51.6
ndhF-rpl32_p2 TCTTCCCTGTCCCAAGCATA AAGTTTCGTTCTCAGTCGGG 50.0 50.0 58.0 57.8 1926-2834 bp 0.181818 51.6
ndhF-rpl32_p3 TATCTTCCCTGTCCCAAGCA TTCGTTCTCAGTCGGGGTAT 50.0 50.0 58.0 58.2 1924-2832 bp 0.181818 51.6
ndhF-rpl32_p4 TCTTCCCTGTCCCAAGCATA TTCGTTCTCAGTCGGGGTAT 50.0 50.0 58.0 58.2 1922-2830 bp 0.181818 51.6
ndhF-rpl32_p5 TCTTCCCTGTCCCAAGCATA TTTCGTTCTCAGTCGGGGTA 50.0 50.0 58.0 58.4 1923-2831 bp 0.181818 49.8