Hotspot

psbM-petN

LSC · 29845-30647 · 803 bp

Back to Cymbidium

Pi 0.024

MarkerSeek score 72.50

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 803
variable_sites: 140
pi: 0.024
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	CTTGCGCTTATTGCTACTGC	TAGTAAGTCTCGCTTGGGCT	50.0	50.0	57.9	58.2	612-1012 bp	1.0	90.0
psbM-petN_p2	CTTGCGCTTATTGCTACTGC	AGGGGACATCATTTGCATGG	50.0	50.0	57.9	58.2	635-1035 bp	1.0	89.5
psbM-petN_p3	CTTGCGCTTATTGCTACTGC	GGGGACATCATTTGCATGGA	50.0	50.0	57.9	58.2	634-1034 bp	1.0	89.5
psbM-petN_p4	ACGTCAAAACAGTCAGCCAA	TAGTAAGTCTCGCTTGGGCT	45.0	50.0	58.3	58.2	551-951 bp	1.0	88.8
psbM-petN_p5	ATTGCTACTGCGCTGTTCAT	TAGTAAGTCTCGCTTGGGCT	45.0	50.0	58.3	58.2	603-1003 bp	1.0	88.7