Hotspot

rpoB-trnC-GCA

LSC · 27216-28682 · 1467 bp

Back to Cymbidium

Pi 0.0371

MarkerSeek score 79.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1467
variable_sites: 268
pi: 0.0371
unique_haplotype_count: 41
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GTGGACATTCCCTCATTTCCA	TAAAGGATTTGCAGTCCCCC	47.6	50.0	57.9	57.5	1311-1562 bp	0.931818	76.4
rpoB-trnC-GCA_p2	TGTGGACATTCCCTCATTTCC	TAAAGGATTTGCAGTCCCCC	47.6	50.0	57.9	57.5	1312-1563 bp	0.931818	76.4
rpoB-trnC-GCA_p3	GTGGACATTCCCTCATTTCCA	CGACACCCAGATTCGAACTG	47.6	55.0	57.9	58.6	1335-1586 bp	0.954545	76.3
rpoB-trnC-GCA_p4	TGTGGACATTCCCTCATTTCC	CGACACCCAGATTCGAACTG	47.6	55.0	57.9	58.6	1336-1587 bp	0.954545	76.3
rpoB-trnC-GCA_p5	CCAGGTATTGTGGACATTCCC	TAAAGGATTTGCAGTCCCCC	52.4	50.0	58.3	57.5	1151-1571 bp	0.977273	76.1