Hotspot

atpH-atpI

LSC · 14256-14839 · 584 bp

Back to Cymbidium

Pi 0.0165

MarkerSeek score 71.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 584
variable_sites: 62
pi: 0.0165
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CGCAATACCTTCTACGGCTT	AACTTTGGCTGCGGCTTATA	50.0	45.0	58.1	57.9	706-830 bp	1.0	89.7
atpH-atpI_p2	TCCGATAGAAGCAAGCCCTA	AACTTTGGCTGCGGCTTATA	50.0	45.0	57.9	57.9	655-779 bp	1.0	89.7
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	AACTTTGGCTGCGGCTTATA	45.0	45.0	57.9	57.9	634-758 bp	1.0	89.3
atpH-atpI_p4	CCGATAGAAGCAAGCCCTAC	AACTTTGGCTGCGGCTTATA	55.0	45.0	57.8	57.9	654-778 bp	1.0	89.0
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	AACTTTGGCTGCGGCTTATA	50.0	45.0	57.8	57.9	633-757 bp	1.0	88.8