Hotspot

trnS-GCU-trnR-UCU

LSC · 9010-10553 · 1544 bp

Back to Cymbidium

Pi 0.025

MarkerSeek score 71.80

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1544
variable_sites: 276
pi: 0.025
unique_haplotype_count: 37
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	CAATCCGACGCTTTAGTCCA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	1530-1739 bp	1.0	75.6
trnS-GCU-trnR-UCU_p2	AATCCGACGCTTTAGTCCAC	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	1529-1738 bp	1.0	75.5
trnS-GCU-trnR-UCU_p3	CAATCCGACGCTTTAGTCCA	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	1536-1745 bp	1.0	74.9
trnS-GCU-trnR-UCU_p4	AATCCGACGCTTTAGTCCAC	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	1535-1744 bp	1.0	74.9
trnS-GCU-trnR-UCU_p5	AGCAATCCGACGCTTTAGTC	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.4	56.9	1532-1741 bp	1.0	72.7