Hotspot

trnK-UUU-rps16

LSC · 4948-5499 · 552 bp

Back to Cymbidium

Pi 0.0464
MarkerSeek score 78.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
552
variable_sites
167
pi
0.0464
unique_haplotype_count
40
primer_available
yes
alignment_reliability
0.93
markerseek_score
78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AGAAAGGAAAAACAAGAATCATGA 50.0 29.2 58.2 55.2 521-799 bp 0.931818 49.3
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AGAAAGGAAAAACAAGAATCATGA 50.0 29.2 58.2 55.2 520-798 bp 0.931818 49.3
trnK-UUU-rps16_p3 TACCGTTGAGTTAGCAACCC AGAAAGGAAAAACAAGAATCATGA 50.0 29.2 57.5 55.2 507-785 bp 0.931818 49.3
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT AAGAAAGGAAAAACAAGAATCATGA 50.0 28.0 58.2 55.9 522-800 bp 0.931818 49.3
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AAGAAAGGAAAAACAAGAATCATGA 50.0 28.0 58.2 55.9 521-799 bp 0.931818 49.3