Hotspot

rps18

LSC · 71117-71560 · 444 bp

Back to Cymbaria

Pi 0.0463

MarkerSeek score 72.90

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 444
variable_sites: 19
pi: 0.0463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	AGAAATAAACTAAACAAACAAACC	TGAATAAAGAATAAGCCTCCCT	25.0	36.4	52.7	54.6	479-495 bp	1.0	47.1
rps18_p2	AGAAATAAACTAAACAAACAAACC	ATGAATAAAGAATAAGCCTCCCT	25.0	34.8	52.7	55.1	480-496 bp	1.0	47.1
rps18_p3	AGAAATAAACTAAACAAACAAACC	TGAATAAAGAATAAGCCTCCC	25.0	38.1	52.7	53.0	479-495 bp	1.0	47.1
rps18_p4	AGAAATAAACTAAACAAACAAACC	AATGAATAAAGAATAAGCCTCCCT	25.0	33.3	52.7	55.9	481-497 bp	1.0	47.1
rps18_p5	AGAAATAAACTAAACAAACAAACC	TCAAATGAATAAAGAATAAGCCTCCC	25.0	34.6	52.7	57.8	484-500 bp	1.0	47.1