Hotspot

rpl32-trnL-UAG

SSC · 114471-115216 · 746 bp

Back to Cymbaria

Pi 0.0615

MarkerSeek score 77.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 746
variable_sites: 44
pi: 0.0615
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCGGGGTATTGGACATCATT	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	928 bp	0.5	59.8
rpl32-trnL-UAG_p2	GCGGGGTATTGGACATCATT	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	929 bp	0.5	59.8
rpl32-trnL-UAG_p3	GCGGGGTATTGGACATCATT	TTAGCCACTCGGATTGAAGC	50.0	50.0	58.0	58.0	1526 bp	0.5	59.7
rpl32-trnL-UAG_p4	GCGGGGTATTGGACATCATT	AAGCGCGGGTACTAAGATTG	50.0	50.0	58.0	58.1	1510 bp	0.5	59.3
rpl32-trnL-UAG_p5	GCGGGGTATTGGACATCATT	ATTGAAGCGCGGGTACTAAG	50.0	50.0	58.0	58.1	1514 bp	0.5	59.3