Hotspot

trnM-CAU-ycf2

IRb · 89664-91970 · 2307 bp

Back to Cymbaria

Pi 0.024

MarkerSeek score 75.20

Primer available yes

Label trnM-CAU-ycf2

Feature Metadata

label_name: trnM-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 2307
variable_sites: 52
pi: 0.024
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	AGACAAAGCCACATGGAGAC	50.0	50.0	58.0	57.8	2244-2384 bp	1.0	84.2
trnM-CAU-ycf2_p2	GTTGGGCGCTTTAACCATTC	GACAAAGCCACATGGAGACT	50.0	50.0	58.0	57.8	2243-2383 bp	1.0	84.2
trnM-CAU-ycf2_p3	AGTTGGGCGCTTTAACCATT	AGACAAAGCCACATGGAGAC	45.0	50.0	58.4	57.8	2245-2385 bp	1.0	81.3
trnM-CAU-ycf2_p4	AGTTGGGCGCTTTAACCATT	GACAAAGCCACATGGAGACT	45.0	50.0	58.4	57.8	2244-2384 bp	1.0	81.3
trnM-CAU-ycf2_p5	TTGGGCGCTTTAACCATTCA	GACAAAGCCACATGGAGACT	45.0	50.0	58.4	57.8	2242-2382 bp	1.0	81.2