Hotspot

psbB-psbT

LSC · 77071-77267 · 197 bp

Back to Cymbaria

Pi 0.0406

MarkerSeek score 72.60

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 197
variable_sites: 8
pi: 0.0406
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GAGCATTCCAAAAACTTGGGG	GAACTTTAGGCGGTTCTCGA	47.6	50.0	58.0	57.6	335-336 bp	1.0	70.7
psbB-psbT_p2	GGAGCATTCCAAAAACTTGGG	GAACTTTAGGCGGTTCTCGA	47.6	50.0	58.0	57.6	336-337 bp	1.0	70.7
psbB-psbT_p3	GCTCAAGTAGAGTTTGGAGCA	GAACTTTAGGCGGTTCTCGA	47.6	50.0	58.0	57.6	351-352 bp	1.0	70.6
psbB-psbT_p4	TGCTCAAGTAGAGTTTGGAGC	GAACTTTAGGCGGTTCTCGA	47.6	50.0	58.0	57.6	352-353 bp	1.0	70.6
psbB-psbT_p5	TCCAAAAACTTGGGGATCCA	GAACTTTAGGCGGTTCTCGA	45.0	50.0	56.9	57.6	329-330 bp	1.0	70.4