Hotspot

trnK-UUU-accD

LSC · 57881-59569 · 1689 bp

Back to Cymbaria

Pi 0.196

MarkerSeek score 86.30

Primer available yes

Label trnK-UUU-accD

Feature Metadata

label_name: trnK-UUU-accD
feature_type: igs
region: LSC
length_bp: 1689
variable_sites: 311
pi: 0.196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 86.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-accD_p1	CTAGTTCCGGGTTCGAATCC	CCTCATGTTTAGGAGTTTTTGACA	55.0	37.5	57.8	57.4	1746-2631 bp	1.0	53.0
trnK-UUU-accD_p2	CTAGTTCCGGGTTCGAATCC	TCCTCATGTTTAGGAGTTTTTGAC	55.0	37.5	57.8	57.1	1747-2632 bp	1.0	51.9
trnK-UUU-accD_p3	ACTCCATCCGACTAGTTCCG	CCTCATGTTTAGGAGTTTTTGACA	55.0	37.5	58.6	57.4	1757-2642 bp	1.0	51.9
trnK-UUU-accD_p4	CTAGTTCCGGGTTCGAATCC	CCTCATGTTTAGGAGTTTTTGACAT	55.0	36.0	57.8	57.8	1746-2631 bp	1.0	51.9
trnK-UUU-accD_p5	CTAGTTCCGGGTTCGAATCC	TCCTCATGTTTAGGAGTTTTTGA	55.0	34.8	57.8	55.8	1747-2632 bp	1.0	51.9