Hotspot

ycf3-psaA

LSC · 16571-17247 · 677 bp

Back to Cymbaria

Pi 0.0726

MarkerSeek score 79.00

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: LSC
length_bp: 677
variable_sites: 48
pi: 0.0726
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	TCACGAGGCGTTTCGAATAA	TGAGAAATGACCCGGTTTGG	45.0	50.0	57.6	58.1	796-817 bp	1.0	82.8
ycf3-psaA_p2	TCACGAGGCGTTTCGAATAA	CCCAGTTCTCGAAAGACGTT	45.0	50.0	57.6	57.8	776-797 bp	1.0	82.5
ycf3-psaA_p3	ATCACGAGGCGTTTCGAATA	TGAGAAATGACCCGGTTTGG	45.0	50.0	57.4	58.1	797-818 bp	1.0	81.0
ycf3-psaA_p4	ATCACGAGGCGTTTCGAATA	CCCAGTTCTCGAAAGACGTT	45.0	50.0	57.4	57.8	777-798 bp	1.0	80.7
ycf3-psaA_p5	TCACGAGGCGTTTCGAATAA	TTTGGCCCAGTTCTCGAAAG	45.0	50.0	57.6	58.4	781-802 bp	1.0	80.5