Hotspot

ycf1

SSC · 123985-129336 · 5352 bp

Back to Cymatocarpus

Pi 0.0153

MarkerSeek score 49.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5352
variable_sites: 122
pi: 0.0153
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGACATTTGTTCGCTGCAAA	TTTAACAGTTTGGGGGACGG	40.0	50.0	56.8	58.0	610 bp	0.666667	57.4
ycf1_p2	AGACATTTGTTCGCTGCAAA	TTCAATTTCCCGAGTGGTCC	40.0	50.0	56.8	57.8	854 bp	0.666667	56.1
ycf1_p3	AGACATTTGTTCGCTGCAAAA	TTTAACAGTTTGGGGGACGG	38.1	50.0	57.6	58.0	610 bp	0.666667	55.2
ycf1_p4	AGACATTTGTTCGCTGCAAA	GGGTTCAACTGTCCGAAACA	40.0	50.0	56.8	58.3	782 bp	0.666667	55.0
ycf1_p5	AGACATTTGTTCGCTGCAAAA	TTCAATTTCCCGAGTGGTCC	38.1	50.0	57.6	57.8	854 bp	0.666667	53.8