Hotspot

clpP

LSC · 69734-71773 · 2040 bp

Back to Cymatocarpus

Pi 0.0152

MarkerSeek score 53.10

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2040
variable_sites: 46
pi: 0.0152
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CCCCCGCTTAATGGATAACC	CATTCAGAAGCCCGTTCGTA	55.0	50.0	58.1	58.0	2803-2828 bp	1.0	81.2
clpP_p2	CCCCCGCTTAATGGATAACC	ATACGCAATGGGGGTGAATC	55.0	50.0	58.1	58.0	2860-2885 bp	1.0	81.0
clpP_p3	CGAGTACATGTTCCTCGTCG	CATTCAGAAGCCCGTTCGTA	55.0	50.0	58.2	58.0	2559-2583 bp	1.0	80.4
clpP_p4	CCGAGTACATGTTCCTCGTC	CATTCAGAAGCCCGTTCGTA	55.0	50.0	57.8	58.0	2560-2584 bp	1.0	80.4
clpP_p5	TCTTTGGCTCTACCCCATGA	CATTCAGAAGCCCGTTCGTA	50.0	50.0	58.0	58.0	2966-2971 bp	0.666667	66.9