Hotspot

ndhE-ndhG

SSC · 118221-118481 · 261 bp

Back to Cymatocarpus

Pi 0.0471

MarkerSeek score 73.50

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 261
variable_sites: 18
pi: 0.0471
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TCGATTGGTTGATGCGAGTT	CTCCTTATTTACGTGGGGGC	45.0	55.0	57.9	58.0	880-895 bp	1.0	82.7
ndhE-ndhG_p2	TCGATTGGTTGATGCGAGTT	TTACGTGGGGGCCATAAATG	45.0	50.0	57.9	57.9	872-887 bp	1.0	82.6
ndhE-ndhG_p3	CGATTGGTTGATGCGAGTTG	CTCCTTATTTACGTGGGGGC	50.0	55.0	57.8	58.0	879-894 bp	1.0	82.2
ndhE-ndhG_p4	TCGATTGGTTGATGCGAGTT	GGAGGTCTGGGAGTGGTATT	45.0	55.0	57.9	58.1	1003-1018 bp	1.0	81.9
ndhE-ndhG_p5	TCGATTGGTTGATGCGAGTT	TTCAATCTGTGGACCGTTGG	45.0	50.0	57.9	58.1	808-814 bp	0.666667	66.0