Hotspot

ndhF-rpl32

SSC · 112609-113437 · 829 bp

Back to Cymatocarpus

Pi 0.0282

MarkerSeek score 66.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 829
variable_sites: 35
pi: 0.0282
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAAAGTCCACATACGCCGAA	ACGATGTCCAATATCCCTTCC	45.0	47.6	57.8	57.2	1040-1062 bp	1.0	69.3
ndhF-rpl32_p2	AAAGTCCACATACGCCGAAG	ACGATGTCCAATATCCCTTCC	50.0	47.6	58.3	57.2	1039-1061 bp	1.0	68.4
ndhF-rpl32_p3	AGAAAAGTCCACATACGCCG	ACGATGTCCAATATCCCTTCC	50.0	47.6	58.3	57.2	1042-1064 bp	1.0	68.4
ndhF-rpl32_p4	GGTCGTGTGAACCAAAAACC	ACGATGTCCAATATCCCTTCC	50.0	47.6	57.8	57.2	1420-1442 bp	1.0	67.9
ndhF-rpl32_p5	TTTTTGACAAGCATTCGCCG	ACGATGTCCAATATCCCTTCC	45.0	47.6	58.2	57.2	1445-1467 bp	1.0	67.6