Hotspot

psbE-petL

LSC · 64280-65564 · 1285 bp

Back to Cymatocarpus

Pi 0.02

MarkerSeek score 66.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1285
variable_sites: 36
pi: 0.02
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	1339-1372 bp	1.0	70.2
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	AGCTGCTAGTAGAAAACCGA	55.0	45.0	58.2	56.3	1334-1367 bp	1.0	69.9
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	AGCTGCTAGTAGAAAACCGA	50.0	45.0	58.4	56.3	1332-1365 bp	1.0	68.7
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AAGCTGCTAGTAGAAAACCGA	50.0	42.9	57.8	57.0	1340-1373 bp	1.0	68.3
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGAA	50.0	42.9	57.8	57.0	1339-1372 bp	1.0	68.3