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petA-psbJ

LSC · 62603-63502 · 900 bp

Back to Cymatocarpus

Pi 0.0282

MarkerSeek score 76.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 900
variable_sites: 37
pi: 0.0282
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGACTTTTGT	GCTGGTATTCTTGTGATCGGT	45.0	47.6	57.6	58.1	1076-1123 bp	1.0	72.3
petA-psbJ_p2	ACGTGTCCAAGGACTTTTGT	AGCTGGTATTCTTGTGATCGG	45.0	47.6	57.6	57.8	1077-1124 bp	1.0	71.5
petA-psbJ_p3	TAGGATCGGTTGTTTTGGCA	GCTGGTATTCTTGTGATCGGT	45.0	47.6	57.4	58.1	1050-1097 bp	1.0	71.1
petA-psbJ_p4	TAGGATCGGTTGTTTTGGCA	AGCTGGTATTCTTGTGATCGG	45.0	47.6	57.4	57.8	1051-1098 bp	1.0	70.4
petA-psbJ_p5	TCCATTACGTGTCCAAGGAC	GCTGGTATTCTTGTGATCGGT	50.0	47.6	57.2	58.1	1082-1129 bp	1.0	69.5