Hotspot

ndhC-trnV-GUA

LSC · 50305-51215 · 911 bp

Back to Cymatocarpus

Pi 0.0206

MarkerSeek score 64.60

Primer available yes

Label ndhC-trnV-GUA

Feature Metadata

label_name: ndhC-trnV-GUA
feature_type: igs
region: LSC
length_bp: 911
variable_sites: 27
pi: 0.0206
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-GUA_p1	GCCCAGAAAATATCATATTCGT	GTTCGAGTCCGTATAGCCCT	36.4	55.0	54.4	58.7	928-967 bp	1.0	46.1
ndhC-trnV-GUA_p2	GCCCAGAAAATATCATATTCGT	TTCGAGTCCGTATAGCCCTA	36.4	50.0	54.4	57.1	927-966 bp	1.0	46.1
ndhC-trnV-GUA_p3	GCCCAGAAAATATCATATTCGT	AGGTCTACGGTTCGAGTCC	36.4	57.9	54.4	58.1	937-976 bp	1.0	46.1
ndhC-trnV-GUA_p4	GCCCAGAAAATATCATATTCGT	TCGAACTCTTTTACCGAGCAG	36.4	47.6	54.4	58.1	956-995 bp	1.0	46.0
ndhC-trnV-GUA_p5	GCCCAGAAAATATCATATTCGT	TCGAACTCTTTTACCGAGCA	36.4	45.0	54.4	56.9	956-995 bp	1.0	46.0