Hotspot

trnT-ACA-trnL-UUA

LSC · 46028-46637 · 610 bp

Back to Cymatocarpus

Pi 0.0251

MarkerSeek score 66.60

Primer available yes

Label trnT-ACA-trnL-UUA

Feature Metadata

label_name: trnT-ACA-trnL-UUA
feature_type: igs
region: LSC
length_bp: 610
variable_sites: 23
pi: 0.0251
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-ACA-trnL-UUA_p1	GCGATGCTCTAACCTCTGAG	CTACCGATTCCGCCATATCC	55.0	55.0	57.9	57.7	662-868 bp	1.0	79.6
trnT-ACA-trnL-UUA_p2	GGAATCGAACCGATGACCAT	CTACCGATTCCGCCATATCC	50.0	55.0	57.8	57.7	694-900 bp	1.0	78.6
trnT-ACA-trnL-UUA_p3	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	55.0	50.0	57.9	57.5	703-909 bp	1.0	77.7
trnT-ACA-trnL-UUA_p4	GGAATCGAACCGATGACCAT	GGTTTCCATACCAAGGCTCA	50.0	50.0	57.8	57.5	735-941 bp	1.0	76.8
trnT-ACA-trnL-UUA_p5	CTAACCTCTGAGCTAAGCGG	CTACCGATTCCGCCATATCC	55.0	55.0	57.5	57.7	654-860 bp	1.0	76.7