Hotspot

trnE-GAA-trnT-ACC

LSC · 30360-31174 · 815 bp

Back to Cymatocarpus

Pi 0.0221

MarkerSeek score 61.20

Primer available yes

Label trnE-GAA-trnT-ACC

Feature Metadata

label_name: trnE-GAA-trnT-ACC
feature_type: igs
region: LSC
length_bp: 815
variable_sites: 27
pi: 0.0221
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-GAA-trnT-ACC_p1	CGTAGACATCTTGCCAACGA	TCGATGGGTTAGGTCCACTT	50.0	50.0	58.0	58.1	1374-1387 bp	1.0	83.6
trnE-GAA-trnT-ACC_p2	CGTAGACATCTTGCCAACGA	TTCGATGGGTTAGGTCCACT	50.0	50.0	58.0	58.1	1375-1388 bp	1.0	83.6
trnE-GAA-trnT-ACC_p3	CGTAGACATCTTGCCAACGA	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	1057-1062 bp	1.0	83.3
trnE-GAA-trnT-ACC_p4	CGTAGACATCTTGCCAACGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	58.0	57.9	1022-1027 bp	1.0	82.6
trnE-GAA-trnT-ACC_p5	CGTAGACATCTTGCCAACGA	CCGATGACTTACGCCTTACC	50.0	55.0	58.0	58.2	1054-1059 bp	1.0	82.1