Hotspot

trnD-GAC-trnY-UAC

LSC · 29683-30143 · 461 bp

Back to Cymatocarpus

Pi 0.0181

MarkerSeek score 64.50

Primer available yes

Label trnD-GAC-trnY-UAC

Feature Metadata

label_name: trnD-GAC-trnY-UAC
feature_type: igs
region: LSC
length_bp: 461
variable_sites: 12
pi: 0.0181
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GAC-trnY-UAC_p1	TATTGAATTCGCCGGGACTG	GCAAGATGTCTACGCTGGTT	50.0	50.0	58.1	58.3	573-585 bp	1.0	81.7
trnD-GAC-trnY-UAC_p2	TATTGAATTCGCCGGGACTG	GTTGGCAAGATGTCTACGCT	50.0	50.0	58.1	58.3	577-589 bp	1.0	81.6
trnD-GAC-trnY-UAC_p3	TATTGAATTCGCCGGGACTG	TTGGCAAGATGTCTACGCTG	50.0	50.0	58.1	58.3	576-588 bp	1.0	81.6
trnD-GAC-trnY-UAC_p4	TATTGAATTCGCCGGGACTG	GGGGACGGACTGTAAATTCG	50.0	55.0	58.1	58.4	596-608 bp	1.0	80.9
trnD-GAC-trnY-UAC_p5	TATTGAATTCGCCGGGACTG	GGGACGGACTGTAAATTCGT	50.0	50.0	58.1	57.6	595-607 bp	1.0	80.7